Research in our lab focuses on deciphering the molecular mechanisms that underlie phosphorylation-dependent signaling in normal cells and tissues and disease. Protein phosphorylation is an essential regulatory mechanism that controls nearly all aspects of cell division and is commonly dysregulated in human cancers. Protein kinases and phosphatases are the direct effectors of phosphorylation signaling. We are using state-of-the-art approaches in proteomics, biochemistry, cell biology, and imaging to functionally dissect signaling networks that are regulated by the interplay of protein kinases and phosphatases. We are developing methods for quantitative mass spectrometry-based proteomics and phosphoproteomics to gain novel insights into these complex signaling networks by determining dynamic changes in protein expression, protein-protein interactions, and post-translational modifications.